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ATCC mouse mesangial glomerulus sv40 mes13 kidney cells
Mouse Mesangial Glomerulus Sv40 Mes13 Kidney Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 128 article reviews

mouse mesangial glomerulus sv40 mes13 kidney cells - by Bioz Stars, 2026-10

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Article Title: Dysregulation of argininosuccinate synthase 1, phosphoenolpyruvate carboxykinase 1, and carbonyl reductase 3: Key arginine-proline metabolic mediators in mesangioproliferative glomerulopathies.
Article Snippet: Aberrant proliferation of mesangial cells (MCs) is a central driver of glomerular injury in IgA nephropathy (IgAN) and lupus nephritis (LN), the two most common mesangioproliferative glomerulopathies.. Emerging evidence implicates dysregulated arginine–proline metabolism in this process; however, the key regulatory genes and underlying mechanisms remain incompletely defined.. Here, we performed integrative bulk RNA sequencing of glomerular datasets and applied machine learning algorithms (LASSO and SVM-RFE) to identify disease-specific differentially expressed genes (DEGs) linked to the arginine–proline metabolic pathway.

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APN evaluation in vitro , with in vivo measurement of IPGTT, ITT, blood glucose and body weight in vivo. (A) C2C12 myotubes viability was assessed using the CCK8 assay . After transfection with APN-mRNA-LNP, the half maximal inhibitory concentration (IC50) was determined after 24 hours. ( B ) Relative APN Expression in differentiated Myotubes cell C2C12, (C) Relative APN Expression in Kidney mesangial cell SV40-MES13. APN gene expression was calculated based on the Law of Fold-change, which is 2 -ΔΔCT . Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro . Values are mean ± SD. n = 9, student t-test. ( D ) APN protein expression, three separate blot analyzes of the samples were conducted. ( E ) Optical densitometry was used to semi-quantify the bands, and ImageJ (1.53t) digital imaging processing software was used for analysis. GAPDH has been used as an endogenous control for protein expression. Protein levels are represented as mean ratio values quantified from protein bands of APN versus GAPDH compared to negative control. (n = 3, Mann-Whitney U tests), Significances are shown above bars: †† P < 0.01 vs. negative control (NC), ** P < 0.01 vs. positive control (PC) “PBS” and ## P < 0.01 vs. Empty-LNP. ( F ) IPGTT with AUC of IPGTT, (G) ITT curve with AUC of ITT. The IPGTT and insulin ITT were utilized at 23 weeks old, to evaluate glucose tolerance and insulin sensitivity, respectively to depict significant changes between groups, the area under the curve (AUC) of IPGTT and ITT was calculated by the trapezoid method. Values are mean ± SD. n = 5 each group, Mann-Whitney U tests, ** P < 0.01 vs. PBS control group, NS; Non-significant. H) Blood glucose levels at different time points were evaluated by glucometer; I) Body weight at different time points. Values are mean ± SD. n = 5 each group, Mann-Whitney U tests, ** P < 0.01 vs. PBS control group and ## P < 0.01 vs. Empty-LNP group.

Journal: Aging and Disease

Article Title: Adiponectin mRNA Conjugated with Lipid Nanoparticles Specifically Targets the Pathogenesis of Type 2 Diabetes

doi: 10.14336/AD.2024.0162

Figure Lengend Snippet: APN evaluation in vitro , with in vivo measurement of IPGTT, ITT, blood glucose and body weight in vivo. (A) C2C12 myotubes viability was assessed using the CCK8 assay . After transfection with APN-mRNA-LNP, the half maximal inhibitory concentration (IC50) was determined after 24 hours. ( B ) Relative APN Expression in differentiated Myotubes cell C2C12, (C) Relative APN Expression in Kidney mesangial cell SV40-MES13. APN gene expression was calculated based on the Law of Fold-change, which is 2 -ΔΔCT . Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro . Values are mean ± SD. n = 9, student t-test. ( D ) APN protein expression, three separate blot analyzes of the samples were conducted. ( E ) Optical densitometry was used to semi-quantify the bands, and ImageJ (1.53t) digital imaging processing software was used for analysis. GAPDH has been used as an endogenous control for protein expression. Protein levels are represented as mean ratio values quantified from protein bands of APN versus GAPDH compared to negative control. (n = 3, Mann-Whitney U tests), Significances are shown above bars: †† P < 0.01 vs. negative control (NC), ** P < 0.01 vs. positive control (PC) “PBS” and ## P < 0.01 vs. Empty-LNP. ( F ) IPGTT with AUC of IPGTT, (G) ITT curve with AUC of ITT. The IPGTT and insulin ITT were utilized at 23 weeks old, to evaluate glucose tolerance and insulin sensitivity, respectively to depict significant changes between groups, the area under the curve (AUC) of IPGTT and ITT was calculated by the trapezoid method. Values are mean ± SD. n = 5 each group, Mann-Whitney U tests, ** P < 0.01 vs. PBS control group, NS; Non-significant. H) Blood glucose levels at different time points were evaluated by glucometer; I) Body weight at different time points. Values are mean ± SD. n = 5 each group, Mann-Whitney U tests, ** P < 0.01 vs. PBS control group and ## P < 0.01 vs. Empty-LNP group.

Article Snippet: SV40 MES13: Mouse mesangial glomerulus SV40-MES13 kidney cells were obtained from the American Type Culture Collection (ATCC CRL-1927) (Manassas, VA).

Techniques: In Vitro, In Vivo, CCK-8 Assay, Transfection, Concentration Assay, Expressing, Gene Expression, Imaging, Software, Control, Negative Control, MANN-WHITNEY, Positive Control

Relative gene expression in different tissues . ( A ) Glut-4 gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. ( B ) DGKd gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, and Kidney. ( C ) PKCe gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. Gene expression was calculated based on the Law of Fold-change, which is 2 -ΔΔCT . Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro. Values are mean ± SD. n = 9, student t-test. In vivo, a total of five biological samples (n = 5) were collected. Of each sample, three replicates were performed technically. ( D ) Glut-4, DGKd, and PKCe protein expression, three separate blot analysis of the samples were conducted. ( E ) Optical densitometry was used to semi-quantify the bands, and ImageJ (1.53t) digital imaging processing software was used for analysis. GAPDH has been used as an endogenous control for protein expression. Protein levels are represented as mean ratio values quantified from protein bands of the targeted protein versus GAPDH compared to negative control. (n = 3, Mann-Whitney U tests), Significances are shown above bars: †† P < 0.01 vs. negative control (NC), ** P < 0.01 vs. positive control (PC) “PBS” and ## P < 0.01 vs. Empty-LNP.

Journal: Aging and Disease

Article Title: Adiponectin mRNA Conjugated with Lipid Nanoparticles Specifically Targets the Pathogenesis of Type 2 Diabetes

doi: 10.14336/AD.2024.0162

Figure Lengend Snippet: Relative gene expression in different tissues . ( A ) Glut-4 gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. ( B ) DGKd gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, and Kidney. ( C ) PKCe gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. Gene expression was calculated based on the Law of Fold-change, which is 2 -ΔΔCT . Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro. Values are mean ± SD. n = 9, student t-test. In vivo, a total of five biological samples (n = 5) were collected. Of each sample, three replicates were performed technically. ( D ) Glut-4, DGKd, and PKCe protein expression, three separate blot analysis of the samples were conducted. ( E ) Optical densitometry was used to semi-quantify the bands, and ImageJ (1.53t) digital imaging processing software was used for analysis. GAPDH has been used as an endogenous control for protein expression. Protein levels are represented as mean ratio values quantified from protein bands of the targeted protein versus GAPDH compared to negative control. (n = 3, Mann-Whitney U tests), Significances are shown above bars: †† P < 0.01 vs. negative control (NC), ** P < 0.01 vs. positive control (PC) “PBS” and ## P < 0.01 vs. Empty-LNP.

Article Snippet: SV40 MES13: Mouse mesangial glomerulus SV40-MES13 kidney cells were obtained from the American Type Culture Collection (ATCC CRL-1927) (Manassas, VA).

Techniques: Gene Expression, Muscles, In Vitro, In Vivo, Expressing, Imaging, Software, Control, Negative Control, MANN-WHITNEY, Positive Control

Relative IR expression in different studied tissues . ( A ) Differentiated Myotubes cell C2C12; (B) Kidney mesangial cell SV40-MES13; (C) Skeletal muscles; (D) Liver; (E) Kidney. IR gene expression was calculated based on the Law of Fold-change, which is 2 -ΔΔCT . Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro . Values are mean ± SD. n = 9, student t-test. In vivo , a total of five biological samples (n = 5, student t-test) were collected. Of each sample, three replicates were performed technically. Significances are shown above bars.

Journal: Aging and Disease

Article Title: Adiponectin mRNA Conjugated with Lipid Nanoparticles Specifically Targets the Pathogenesis of Type 2 Diabetes

doi: 10.14336/AD.2024.0162

Figure Lengend Snippet: Relative IR expression in different studied tissues . ( A ) Differentiated Myotubes cell C2C12; (B) Kidney mesangial cell SV40-MES13; (C) Skeletal muscles; (D) Liver; (E) Kidney. IR gene expression was calculated based on the Law of Fold-change, which is 2 -ΔΔCT . Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro . Values are mean ± SD. n = 9, student t-test. In vivo , a total of five biological samples (n = 5, student t-test) were collected. Of each sample, three replicates were performed technically. Significances are shown above bars.

Article Snippet: SV40 MES13: Mouse mesangial glomerulus SV40-MES13 kidney cells were obtained from the American Type Culture Collection (ATCC CRL-1927) (Manassas, VA).

Techniques: Expressing, Muscles, Gene Expression, In Vitro, In Vivo

EGFR expression in vitro and in vivo . (A) Relative EGFR Expression in different Differentiated Myotubes cell C2C12; (B) Kidney mesangial cell SV40-MES13; (C) kidney. EGFR gene expression was calculated based on the Law of Fold-change, which is 2 -ΔΔCT . Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro . Values are mean ± SD. n = 9, student t-test. In vivo , a total of five biological samples (n = 5, student t-test) were collected. Of each sample, three replicates were performed technically. Significances are shown above bars. ( D ) Overall correlation between APN and EGFR gene expression in kidney. ( E ) Light microscopic appearance in kidney tissue section of the studied groups of DIO mice, (yellow arrow) indicates Hyperplasia of partial cells in the Bowmans capsule of the glomeruli and (Black arrow) indicates mild diffuse mesangial matrix expansion (PAS stain, 1000X). A total of five biological samples (n = 5, student t-test) were collected; three sections were taken from each sample. Scale bar= 50 μm.

Journal: Aging and Disease

Article Title: Adiponectin mRNA Conjugated with Lipid Nanoparticles Specifically Targets the Pathogenesis of Type 2 Diabetes

doi: 10.14336/AD.2024.0162

Figure Lengend Snippet: EGFR expression in vitro and in vivo . (A) Relative EGFR Expression in different Differentiated Myotubes cell C2C12; (B) Kidney mesangial cell SV40-MES13; (C) kidney. EGFR gene expression was calculated based on the Law of Fold-change, which is 2 -ΔΔCT . Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro . Values are mean ± SD. n = 9, student t-test. In vivo , a total of five biological samples (n = 5, student t-test) were collected. Of each sample, three replicates were performed technically. Significances are shown above bars. ( D ) Overall correlation between APN and EGFR gene expression in kidney. ( E ) Light microscopic appearance in kidney tissue section of the studied groups of DIO mice, (yellow arrow) indicates Hyperplasia of partial cells in the Bowmans capsule of the glomeruli and (Black arrow) indicates mild diffuse mesangial matrix expansion (PAS stain, 1000X). A total of five biological samples (n = 5, student t-test) were collected; three sections were taken from each sample. Scale bar= 50 μm.

Article Snippet: SV40 MES13: Mouse mesangial glomerulus SV40-MES13 kidney cells were obtained from the American Type Culture Collection (ATCC CRL-1927) (Manassas, VA).

Techniques: Expressing, In Vitro, In Vivo, Gene Expression, Staining

Relative gene expression of the pro-inflammatory cytokines . ( A ) TNFa gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. ( B ) IL-6 gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. ( C ) IL-1b gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. Gene expression was calculated based on the Law of Fold-change, which is 2 -ΔΔCT . Both biological and technical triplicates were performed in vitro . Values are mean ± SD. n = 9, student t-test. In vivo , a total of five biological samples (n = 5) were collected. Of each sample, three replicates were performed technically. ( D ) Glut-4 TNFa and IL-6 protein expression, three separate blot analysis of the samples were conducted. ( E ) Optical densitometry was used to semi-quantify the bands, and ImageJ (1.53t) digital imaging processing software was used for analysis. GAPDH has been used as an endogenous control for protein expression. Protein levels are represented as mean ratio values quantified from protein bands of the targeted protein versus GAPDH compared to negative control. (n = 3, Mann-Whitney U tests), Significances are shown above bars: †† P < 0.01 vs. negative control (NC), ** P < 0.01 vs. positive control (PC) “PBS” and ## P < 0.01 vs. Empty-LNP.

Journal: Aging and Disease

Article Title: Adiponectin mRNA Conjugated with Lipid Nanoparticles Specifically Targets the Pathogenesis of Type 2 Diabetes

doi: 10.14336/AD.2024.0162

Figure Lengend Snippet: Relative gene expression of the pro-inflammatory cytokines . ( A ) TNFa gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. ( B ) IL-6 gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. ( C ) IL-1b gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. Gene expression was calculated based on the Law of Fold-change, which is 2 -ΔΔCT . Both biological and technical triplicates were performed in vitro . Values are mean ± SD. n = 9, student t-test. In vivo , a total of five biological samples (n = 5) were collected. Of each sample, three replicates were performed technically. ( D ) Glut-4 TNFa and IL-6 protein expression, three separate blot analysis of the samples were conducted. ( E ) Optical densitometry was used to semi-quantify the bands, and ImageJ (1.53t) digital imaging processing software was used for analysis. GAPDH has been used as an endogenous control for protein expression. Protein levels are represented as mean ratio values quantified from protein bands of the targeted protein versus GAPDH compared to negative control. (n = 3, Mann-Whitney U tests), Significances are shown above bars: †† P < 0.01 vs. negative control (NC), ** P < 0.01 vs. positive control (PC) “PBS” and ## P < 0.01 vs. Empty-LNP.

Article Snippet: SV40 MES13: Mouse mesangial glomerulus SV40-MES13 kidney cells were obtained from the American Type Culture Collection (ATCC CRL-1927) (Manassas, VA).

Techniques: Gene Expression, Muscles, In Vitro, In Vivo, Expressing, Imaging, Software, Control, Negative Control, MANN-WHITNEY, Positive Control

Figure 1. APN evaluation in vitro, with in vivo measurement of IPGTT, ITT, blood glucose and body weight in vivo. (A) C2C12 myotubes viability was assessed using the CCK8 assay. After transfection with APN-mRNA-LNP, the half maximal inhibitory concentration (IC50) was determined after 24 hours. (B) Relative APN Expression in differentiated Myotubes cell C2C12, (C) Relative APN Expression in Kidney mesangial cell SV40-MES13. APN gene expression was calculated based on the Law of Fold- change, which is 2−ΔΔCT. Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro. Values are mean ± SD. n = 9, student t-test. (D) APN protein expression, three separate blot analyzes of the samples were conducted. (E) Optical densitometry was used to semi-quantify the bands, and ImageJ (1.53t) digital imaging processing software was used for analysis. GAPDH has been used as an endogenous control for protein expression. Protein levels are represented as mean ratio values quantified from protein bands of APN versus GAPDH compared to negative control. (n = 3, Mann-Whitney U tests), Significances are shown above bars: ††P < 0.01 vs. negative control (NC), **P < 0.01 vs. positive control (PC) “PBS” and ##P < 0.01 vs. Empty-LNP. (F) IPGTT with AUC of IPGTT, (G) ITT curve with AUC of ITT. The IPGTT and insulin ITT were utilized at 23 weeks old, to evaluate glucose tolerance and insulin sensitivity, respectively to depict significant changes between groups, the area under the curve (AUC) of IPGTT and ITT was calculated by the trapezoid method. Values are mean ± SD. n = 5 each group, Mann-Whitney U tests, ** P < 0.01 vs. PBS control group, NS; Non-significant. H) Blood glucose levels at different time points were evaluated by glucometer; I) Body weight at different time points. Values are mean ± SD. n = 5 each group, Mann-Whitney U tests, ** P < 0.01 vs. PBS control group and ##P < 0.01 vs. Empty-LNP group.

Journal: Aging and disease

Article Title: Adiponectin mRNA Conjugated with Lipid Nanoparticles Specifically Targets the Pathogenesis of Type 2 Diabetes

doi: 10.14336/ad.2024.0162

Figure Lengend Snippet: Figure 1. APN evaluation in vitro, with in vivo measurement of IPGTT, ITT, blood glucose and body weight in vivo. (A) C2C12 myotubes viability was assessed using the CCK8 assay. After transfection with APN-mRNA-LNP, the half maximal inhibitory concentration (IC50) was determined after 24 hours. (B) Relative APN Expression in differentiated Myotubes cell C2C12, (C) Relative APN Expression in Kidney mesangial cell SV40-MES13. APN gene expression was calculated based on the Law of Fold- change, which is 2−ΔΔCT. Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro. Values are mean ± SD. n = 9, student t-test. (D) APN protein expression, three separate blot analyzes of the samples were conducted. (E) Optical densitometry was used to semi-quantify the bands, and ImageJ (1.53t) digital imaging processing software was used for analysis. GAPDH has been used as an endogenous control for protein expression. Protein levels are represented as mean ratio values quantified from protein bands of APN versus GAPDH compared to negative control. (n = 3, Mann-Whitney U tests), Significances are shown above bars: ††P < 0.01 vs. negative control (NC), **P < 0.01 vs. positive control (PC) “PBS” and ##P < 0.01 vs. Empty-LNP. (F) IPGTT with AUC of IPGTT, (G) ITT curve with AUC of ITT. The IPGTT and insulin ITT were utilized at 23 weeks old, to evaluate glucose tolerance and insulin sensitivity, respectively to depict significant changes between groups, the area under the curve (AUC) of IPGTT and ITT was calculated by the trapezoid method. Values are mean ± SD. n = 5 each group, Mann-Whitney U tests, ** P < 0.01 vs. PBS control group, NS; Non-significant. H) Blood glucose levels at different time points were evaluated by glucometer; I) Body weight at different time points. Values are mean ± SD. n = 5 each group, Mann-Whitney U tests, ** P < 0.01 vs. PBS control group and ##P < 0.01 vs. Empty-LNP group.

Article Snippet: SV40 MES13: Mouse mesangial glomerulus SV40MES13 kidney cells were obtained from the American Type Culture Collection (ATCC CRL-1927) (Manassas, VA).

Techniques: In Vitro, In Vivo, CCK-8 Assay, Transfection, Concentration Assay, Expressing, Gene Expression, Imaging, Software, Control, Negative Control, MANN-WHITNEY, Positive Control

Figure 3. Relative gene expression in different tissues. (A) Glut-4 gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. (B) DGKd gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, and Kidney. (C) PKCe gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. Gene expression was calculated based on the Law of Fold-change, which is 2−ΔΔCT. Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro. Values are mean ± SD. n = 9, student t-test. In vivo, a total of five biological samples (n = 5) were collected. Of each sample, three replicates were performed technically. (D) Glut-4, DGKd, and PKCe protein expression, three separate blot analysis of the samples were conducted. (E) Optical densitometry was used to semi-quantify the bands, and ImageJ (1.53t) digital imaging processing software was used for analysis. GAPDH has been used as an endogenous control for protein expression. Protein levels are represented as mean ratio values quantified from protein bands of the targeted protein versus GAPDH compared to negative control. (n = 3, Mann-Whitney U tests), Significances are shown above bars: ††P < 0.01 vs. negative control (NC), **P < 0.01 vs. positive control (PC) “PBS” and ##P < 0.01 vs. Empty-LNP.

Journal: Aging and disease

Article Title: Adiponectin mRNA Conjugated with Lipid Nanoparticles Specifically Targets the Pathogenesis of Type 2 Diabetes

doi: 10.14336/ad.2024.0162

Figure Lengend Snippet: Figure 3. Relative gene expression in different tissues. (A) Glut-4 gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. (B) DGKd gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, and Kidney. (C) PKCe gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. Gene expression was calculated based on the Law of Fold-change, which is 2−ΔΔCT. Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro. Values are mean ± SD. n = 9, student t-test. In vivo, a total of five biological samples (n = 5) were collected. Of each sample, three replicates were performed technically. (D) Glut-4, DGKd, and PKCe protein expression, three separate blot analysis of the samples were conducted. (E) Optical densitometry was used to semi-quantify the bands, and ImageJ (1.53t) digital imaging processing software was used for analysis. GAPDH has been used as an endogenous control for protein expression. Protein levels are represented as mean ratio values quantified from protein bands of the targeted protein versus GAPDH compared to negative control. (n = 3, Mann-Whitney U tests), Significances are shown above bars: ††P < 0.01 vs. negative control (NC), **P < 0.01 vs. positive control (PC) “PBS” and ##P < 0.01 vs. Empty-LNP.

Article Snippet: SV40 MES13: Mouse mesangial glomerulus SV40MES13 kidney cells were obtained from the American Type Culture Collection (ATCC CRL-1927) (Manassas, VA).

Techniques: Gene Expression, Muscles, In Vitro, In Vivo, Expressing, Imaging, Software, Control, Negative Control, MANN-WHITNEY, Positive Control

Figure 4. Relative IR expression in different studied tissues. (A) Differentiated Myotubes cell C2C12; (B) Kidney mesangial cell SV40-MES13; (C) Skeletal muscles; (D) Liver; (E) Kidney. IR gene expression was calculated based on the Law of Fold-change, which is 2−ΔΔCT. Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro. Values are mean ± SD. n = 9, student t-test. In vivo, a total of five biological samples (n = 5, student t-test) were collected. Of each sample, three replicates were performed technically. Significances are shown above bars.

Journal: Aging and disease

Article Title: Adiponectin mRNA Conjugated with Lipid Nanoparticles Specifically Targets the Pathogenesis of Type 2 Diabetes

doi: 10.14336/ad.2024.0162

Figure Lengend Snippet: Figure 4. Relative IR expression in different studied tissues. (A) Differentiated Myotubes cell C2C12; (B) Kidney mesangial cell SV40-MES13; (C) Skeletal muscles; (D) Liver; (E) Kidney. IR gene expression was calculated based on the Law of Fold-change, which is 2−ΔΔCT. Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro. Values are mean ± SD. n = 9, student t-test. In vivo, a total of five biological samples (n = 5, student t-test) were collected. Of each sample, three replicates were performed technically. Significances are shown above bars.

Article Snippet: SV40 MES13: Mouse mesangial glomerulus SV40MES13 kidney cells were obtained from the American Type Culture Collection (ATCC CRL-1927) (Manassas, VA).

Techniques: Expressing, Muscles, Gene Expression, In Vitro, In Vivo

Figure 6. EGFR expression in vitro and in vivo. (A) Relative EGFR Expression in different Differentiated Myotubes cell C2C12; (B) Kidney mesangial cell SV40-MES13; (C) kidney. EGFR gene expression was calculated based on the Law of Fold-change, which is 2−ΔΔCT. Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro. Values are mean ± SD. n = 9, student t-test. In vivo, a total of five biological samples (n = 5, student t-test) were collected. Of each sample, three replicates were performed technically. Significances are shown above bars. (D) Overall correlation between APN and EGFR gene expression in kidney. (E) Light microscopic appearance in kidney tissue section of the studied groups of DIO mice, (yellow arrow) indicates Hyperplasia of partial cells in the Bowmans capsule of the glomeruli and (Black arrow) indicates mild diffuse mesangial matrix expansion (PAS stain, 1000X). A total of five biological samples (n = 5, student t-test) were collected; three sections were taken from each sample. Scale bar= 50 μm.

Journal: Aging and disease

Article Title: Adiponectin mRNA Conjugated with Lipid Nanoparticles Specifically Targets the Pathogenesis of Type 2 Diabetes

doi: 10.14336/ad.2024.0162

Figure Lengend Snippet: Figure 6. EGFR expression in vitro and in vivo. (A) Relative EGFR Expression in different Differentiated Myotubes cell C2C12; (B) Kidney mesangial cell SV40-MES13; (C) kidney. EGFR gene expression was calculated based on the Law of Fold-change, which is 2−ΔΔCT. Fold-change values less than one indicate downregulation, whereas values greater than one indicate upregulation. Both biological and technical triplicates were performed in vitro. Values are mean ± SD. n = 9, student t-test. In vivo, a total of five biological samples (n = 5, student t-test) were collected. Of each sample, three replicates were performed technically. Significances are shown above bars. (D) Overall correlation between APN and EGFR gene expression in kidney. (E) Light microscopic appearance in kidney tissue section of the studied groups of DIO mice, (yellow arrow) indicates Hyperplasia of partial cells in the Bowmans capsule of the glomeruli and (Black arrow) indicates mild diffuse mesangial matrix expansion (PAS stain, 1000X). A total of five biological samples (n = 5, student t-test) were collected; three sections were taken from each sample. Scale bar= 50 μm.

Article Snippet: SV40 MES13: Mouse mesangial glomerulus SV40MES13 kidney cells were obtained from the American Type Culture Collection (ATCC CRL-1927) (Manassas, VA).

Techniques: Expressing, In Vitro, In Vivo, Gene Expression, Staining

Figure 7. Relative gene expression of the pro-inflammatory cytokines. (A) TNFa gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. (B) IL-6 gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. (C) IL-1b gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. Gene expression was calculated based on the Law of Fold-change, which is 2−ΔΔCT. Both biological and technical triplicates were performed in vitro. Values are mean ± SD. n = 9, student t-test. In vivo, a total of five biological samples (n = 5) were collected. Of each sample, three replicates were performed technically. (D) Glut-4 TNFa and IL-6 protein expression, three separate blot analysis of the samples were conducted. (E) Optical densitometry was used to semi-quantify the bands, and ImageJ (1.53t) digital imaging processing software was used for analysis. GAPDH has been used as an endogenous control for protein expression. Protein levels are represented as mean ratio values quantified from protein bands of the targeted protein versus GAPDH compared to negative control. (n = 3, Mann-Whitney U tests), Significances are shown above bars: ††P < 0.01 vs. negative control (NC), **P < 0.01 vs. positive control (PC) “PBS” and ##P < 0.01 vs. Empty-LNP.

Journal: Aging and disease

Article Title: Adiponectin mRNA Conjugated with Lipid Nanoparticles Specifically Targets the Pathogenesis of Type 2 Diabetes

doi: 10.14336/ad.2024.0162

Figure Lengend Snippet: Figure 7. Relative gene expression of the pro-inflammatory cytokines. (A) TNFa gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. (B) IL-6 gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. (C) IL-1b gene in differentiated myotubes cell C2C12, Kidney mesangial cell SV40-MES13, Skeletal muscles, Liver, Kidney, White fats, and Brown fats. Gene expression was calculated based on the Law of Fold-change, which is 2−ΔΔCT. Both biological and technical triplicates were performed in vitro. Values are mean ± SD. n = 9, student t-test. In vivo, a total of five biological samples (n = 5) were collected. Of each sample, three replicates were performed technically. (D) Glut-4 TNFa and IL-6 protein expression, three separate blot analysis of the samples were conducted. (E) Optical densitometry was used to semi-quantify the bands, and ImageJ (1.53t) digital imaging processing software was used for analysis. GAPDH has been used as an endogenous control for protein expression. Protein levels are represented as mean ratio values quantified from protein bands of the targeted protein versus GAPDH compared to negative control. (n = 3, Mann-Whitney U tests), Significances are shown above bars: ††P < 0.01 vs. negative control (NC), **P < 0.01 vs. positive control (PC) “PBS” and ##P < 0.01 vs. Empty-LNP.

Article Snippet: SV40 MES13: Mouse mesangial glomerulus SV40MES13 kidney cells were obtained from the American Type Culture Collection (ATCC CRL-1927) (Manassas, VA).

Techniques: Gene Expression, Muscles, In Vitro, In Vivo, Expressing, Imaging, Software, Control, Negative Control, MANN-WHITNEY, Positive Control

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